1.Yongping Ke?, Dali Kong?, Wenxin Zhang?, Wenjie Zeng, Zhe Kong, Xiaofei Dang, Jian-Kang Zhu,Daisuke Miki*,Precise knock-in ofstress-responsivecis-regulatory elements using gene targeting for improving abiotic stress tolerance in plants.New Phytologist,(2025)(?These authors contributed equally to this work; * Corresponding author)
2.Yiqiu Cheng, Lei Zhang, Yongping Ke, Xiaofei Dang,Daisuke Miki*,Double step screening using endogenous marker improves relative gene targeting efficiency in Arabidopsis.Scientific Reports,14: 30791. (2024)(* Corresponding author)
3.Jing Li?, Qi Wei?, Yiqiu Cheng, Dali Kong, Zhe Kong, Yongping Ke, Xiaofei Dang, Jian-Kang Zhu, Hiroaki Shimada,Daisuke Miki*,Cas12a-mediated gene targeting by sequential transformation strategy in Arabidopsis thaliana.BMCPlant Biology,24: 665. (2024)(?These authors contributed equally to this work; * Corresponding author)
4.Yiqiu Cheng, Lei Zhang, Jing Li, Xiaofei Dang, Jian-Kang Zhu, Hiroaki Shimada,Daisuke Miki*,Simple promotion of Cas9 and Cas12a expression improves gene targeting via an all-in-one strategy.Frontiers in Plant Science,15: 1360925. (2024)(* Corresponding author)
5.Jing Li?, Dali Kong?, Yongping Ke, Wenjie Zeng,Daisuke Miki*,Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis.BMC Biology,22(1): 6. (2024)(?These authors contributed equally to this work; * Corresponding author)
6.Wenxin Zhang?, Rui Wang?, Dali Kong?, Fangnan Peng?, Mei Chen?, Wenjie Zeng, Francesca Giaume, Sheng He, Hui Zhang, Zhen Wang, Junko Kyozuka, Jian-Kang Zhu, Fabio Fornara,Daisuke Miki*,Precise and heritable gene targeting in rice using a sequential transformation strategy.Cell Reports Methods,3(1): 100389. (2023)(?These authors contributed equally to this work; * Corresponding author)
7.Zhengjing Zhang?, Wenjie Zeng?, Wenxin Zhang?, Jing Li, Dali Kong, Lei Zhang, Rui Wang, Fangnan Peng, Zhe Kong, Yongping Ke, Heng Zhang, Chanhong Kim, Huiming Zhang, Jose Ramón Botella, Jian-Kang Zhu,Daisuke Miki*,Insights into the molecular mechanisms of CRISPR/Cas9-mediated gene targeting at multiple loci in Arabidopsis.Plant Physiology,190(4): 2203-2216. (2022)(?These authors contributed equally to this work; * Corresponding author)
8.Yi-Zhe Zhang?, Juncheng Lin?, Zhizhong Ren?, Chun-Xiang Chen?,Daisuke Miki?, Si-Si Xie, Jian Zhang, Ya-Nan Chang, Jing Jing, Jun Yan, Qingshun Q. Li,Jian-Kang Zhu, Cheng-Guo Duan,Genome-wide distribution and functions of the AAE complex in epigenetic regulation inArabidopsis.Journal of Integrative Plant Biology,63(4): 707-722. (2021)(?These authors contributed equally to this work)
9.Wenjie Zeng?, Huan Huang?, Xueqiang Lin, Chen Zhu, Ken-Ichi Kosami, Chaofeng Huang, Huiming Zhang, Cheng-Guo Duan, Jian-Kang Zhu,Daisuke Miki*,Roles of DEMETER in regulating DNA methylation in vegetative tissues and pathogen resistance.Journal of Integrative Plant Biology,63(4): 691-706. (2021)(?These authors contributed equally to this work; * Corresponding author)
10.Fangnan Peng?, Wenxin Zhang?, Wenjie Zeng, Jian-Kang Zhu,Daisuke Miki*,Gene targeting inArabidopsisvia an all-in-one strategy that uses a translational enhancer to aid Cas9 expression.Plant Biotechnology Journal,18(4): 892-894. (2020)(?These authors contributed equally to this work; * Corresponding author)
11.Daisuke Miki?*, Wenxin Zhang?, Wenjie Zeng, Zhengyan Feng, Jian-Kang Zhu*,CRISPR/Cas9-mediated gene targeting inArabidopsisusing sequential transformation.NatureCommunications, 9(1): 1967. (2018)(?These authors contributed equally to this work; * Corresponding authors)
12.Daisuke Miki?*, Peiying Zhu?, Wencan Zhang, Yanfei Mao, Zhengyan Feng, Huan Huang, Hui Zhang, Yanqiang Li, Renyi Liu, Huiming Zhang, Yijun Qi, Jian-Kang Zhu,Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway.Scientific Reports, 7(1): 301. (2017)(?These authors contributed equally to this work; * Corresponding author)
13.Weiqiang Qian?,Daisuke Miki?, Mingguang Lei?, Xiaohong Zhu, Huiming Zhang, Yunhua Liu, Yan Li, Zhaobo Lang, Jing Wang, Kai Tang, Renyi Liu, Jian-Kang Zhu,Regulation of Active DNA Demethylation by An α-Crystallin Domain Protein inArabidopsis.Molecular Cell, 55(3): 361-71. (2014)(?These authors contributed equally to this work)
14.Weiqiang Qian?,Daisuke Miki?, Heng Zhang, Yunhua Liu, Xi Zhang, Kai Tang, Yunchao Kan, Honggui La,Xiaojie Li, Shaofang Li, Xiaohong Zhu, Xiaobing Shi, Kangling Zhang, Olga Pontes, Xuemei Chen, Renyi Liu, Zhizhong Gong, Jian-Kang Zhu,A Histone Acetyltransferase Regulates Active DNA Demethylation inArabidopsis.Science, 336(6087): 1445-48. (2012)(?These authors contributed equally to this work)
15.María Isabel Martínez-Macías?, Weiqiang Qian?,Daisuke Miki?, Olga Pontes, Yunhua Liu, Kai Tang, Renyi Liu, Teresa Morales-Ruiz, Rafael R. Ariza, Teresa Roldán-Arjona, Jian-Kang Zhu,A DNA 3’-phosphatase functions in active DNA demethylation inArabidopsis.Molecular Cell, 45(3): 357-70. (2012)(?These authors contributed equally to this work)
16.Daisuke Miki, Ko Shimamoto,De novoDNA methylation induced by siRNA targeted to endogenous transcribed sequences is gene-specific andOsMet1-independent in rice.ThePlant Journal,56(4): 539-49. (2008)
17.Daisuke Miki, Rika Itoh, Ko Shimamoto,RNA silencing of single and multiple members in a gene family of rice.Plant Physiology,138(4): 1903-13. (2005)
18.Daisuke Miki, Ko Shimamoto,Simple RNAi vectors for stable and transient suppression of gene function in rice.Plant and Cell Physiology,45(4):490-495. (2004)
